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Jamroy M, Kudacik-Kramarczyk S, Drabczyk A, Krzan M (2024) Advanced drug carriers: a review of selected protein, polysaccharide, and lipid drug delivery platforms

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Subclinical vitamin C deficiency can show up as newly-onset or worsening seasonal allergies, bleeding gums, dark under-eye circles, low blood sugar spells, worsening depression, broken sleep (from high histamine levels), broken blood vessels (that look like little red or blue dots on the skin), loss of skin elasticity (because you need vitamin C to make collagen), and easy bruising

A sucrose gradient (18% (w/v), 20%, 23%, 25% each 10 ml) was added using the underlayering step gradient technique