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These include vaccine-based and immune-stimulatory approaches evaluated in the adjuvant setting, such as booster peptide vaccination in previously vaccinated patients with resected melanoma (NCT01989559, completed) and a randomized phase III trial testing sargramostim (GM-CSF), peptide vaccination, or their combination following surgical resection of high-risk disease (NCT01989572, completed)

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Briefly, the frozen cortical samples were homogenized in SDT-lysis buffer (100 mM TrisHCl (pH 7.6) containing 0.1 M DTT) using an ultrasonic homogenizer, and the homogenates were heated at 95 C for 3 min