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Science 375(6586):12541261 Li Y et al (2023) Iron and copper: critical executioners of ferroptosis, cuproptosis and other forms of cell death

Lysates of Raw264.7 cells were prepared with lysis buffer containing 50 mM Tris-HCl (pH 7.5), 1 mM EDTA, 10% glycerol, 150 mM NaCl, 1% NP-40, and protease/phosphatase inhibitors (Thermo Fisher Scientific) and incubated with the peptide-conjugate resins for 1 h at 4C

The requirement of long-term patience (3 to 6 months for visible results)

Differences in metabolic profiles at baseline and post-treatment were noted between participants who remitted (HRSD 17 7) and those who gained no meaningful benefits ( 14 on HRSD 17 were eligible for inclusion in the trial