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After cultured for 25 min at 37C, the cells were gently washed with DMEM and digested with 500 l trypsin without EDTA for 3 min at 37C

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Marianna University School of Medicine, 26 Osaka Metropolitan University, 27 Nippon Medical School Chiba Hobusoh Hospital, 28 Texas GI Alliance, 29 National University Hospital, Singapore, 30 Wong Clinics, San Francisco, 31 Kitasato University School of Medicine, 32 National University Health System, Singapore, 33 Nippon Medical School Background: Many patients with chronic hepatitis B (CHB) are in the indeterminate phase, but they make up a diverse cohort with likely different natural history and outcome