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Genome-wide CRISPR/Cas9 screen: Palbociclib sensitization and resistance A doxycycline-inducible Cas9-expressing MCF-7 iCas9 clone was generated by transduction of MCF-7 cells with the Edit-R Inducible Lentiviral hEF1a-Blast-Cas9 Nuclease (Dharmacon) and selected in 10 g/ml blasticidin for 5 days (MCF-7iCas9)