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Astrocytes to the contrary have high cytosolic Cl (3050 mM) 70,72 , and when astrocytic Cl channels are opened (incidentally, astrocytic perisynaptic membranes contain GABA A receptors) Cl efflux is generated to maintain high extracellular Cl concentration and sustain inhibitory transmission 70

S32pH4-5For example, before performing S3, the intermediate 2 is quenched with water, the pH is adjusted to 4-5, the organic phase is collected, dried and concentrated

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But its important to note that other medical conditions can show similar symptoms