glutathione oxidative stress and neurodegeneration Glutathione, Neurodegenerative Diseases: Potential Effect of

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Increased intracellular iron levels and lipid peroxidation are also implicated in tissue damage 21

Cells were collected by centrifugation at 5,000xg for 10 minutes and frozen before resuspension in 150 mL lysis buffer (50 mM Tris-HCl pH 8.5, 500 mM NaCl, 10% glycerol), supplemented with protease inhibitor cocktail (Roche)