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Use water bath to control the reaction temperature at 35 C

The couple broke up several weeks afterward, but later reconciled

Abnormal Ca 2+ handling led to mitochondrial membrane permeabilization with consequent release of either mtDAMPs and MDVs to promote and sustain the inflammation or cytochrome c and pro-apoptotic factors to active caspases and trigger apoptosis, exacerbating the disease progression [24,25,26]