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Once the samples were dried, transmission electron microscopy (JEM-1400Plus, Japan) was used to observe the ultrastructure of the tissue mitochondria to determine the occurrence and severity of ferroptosis

In a study by Kim et al., the ultrasound-sensitive liposomes exhibited a size distribution of 81.94 nm, with a doxorubicin entrapment effectiveness of 97.1 1.44%

RNA isolation and RT-qPCR Total RNA was extracted from pooled retinal tissue from 2 eyes of a single adult fish (individual fish served as a biological replicate) using the RNeasy Plus Mini Kit (Qiagen, Germantown, MD) according to the manufacturers protocol for animal tissue

In the model with insomnia induced by PCPA shows the fissure in the tissues