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Peptide samples were separated with a 60 min linear gradient from 5% to 40% ACN in 15 mM ammonium hydroxide at a flow rate of 0.4 mL/min

After incubation, 20 L of H 2 O 2 substrate solution with or without 5 mM diethylmaleate (DEM) was added to each well, and cells were incubated again for 4 hours at 37C

10 Conclusions Despite numerous research studies and resources invested, the question of what determines the transition from pharyngeal colonization to IMD, which has been raised since Weichselbaums meningococcus, the main agent of epidemic cerebrospinal meningitis, was identified (Weichselbaum, 1887), remains without a definitive answer

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