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Thurston L, et al

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Samples were adjusted with 25 l 10 x lysis buffer (final concentrations 4% SDS, 100 mM HEPES, pH 8.5, 50 mM DTT) and homogenized using a Precellys 24 tissue homogenizer (Bertin Technologies) for 3 cycles (6000 mixes for 20 s followed by 30 s pause)