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Morphological changes, occurring during the activation of microglia and astrocytes, or higher proliferation of NG2 glia, microglia and astrocytes are all hallmarks of ischemia (Davalos et al., 2005

Maintenance of cells and animals Ea.hy926 (E9) cells: E9 cells were purchased from National Centre for Cell Science (NCCS), Pune and maintained in Dulbeccos Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS) and 1% antibiotic-antimycotic solution (Himedia, A002) (10,000 units of penicillin and 10 mg of streptomycin in 0.9% NaCl) in a humidified atmosphere with 5% CO 2 at 37 C

This approach moves beyond non-specific chemotherapy combinations towards mechanism-informed drug partnerships [15]

The samples were loaded on the 96-well S-Trap plate (Protifi), placed on top of a deepwell plate, and centrifuged for 2 min at 1,500 x g at RT