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Molecular docking and dynamics simulation predicted the binding energy and stability of these constituents to UA related transporters

[DOI] [PMC free article] [PubMed] [Google Scholar] 45.Zahran AM, Nafady-Hego H, Moeen SM, Eltyb HA, Wahman MM, Nafady A

44 ClinicalTrials.gov (2021c)

Luciferase reporter assays A bicistronic vector pcDNA3 RLuc POLIRES FLuc (empty reporter) (Addgene, 45642) and pcDNA3 RLuc POLIRES FLuc containing human PRKAA1 5UTR upstream of FLuc (5UTR reporter) were transfected into shCtrl and shGDH1 or shYBX1 Huh7 cells using Lipofectamine 2000 (Thermo Fisher)