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The inserts were removed, the wells were washed with phenol red-free DMEM/HAMs F-12 medium supplemented with 5% FBS and the uptake of nanocarriers in the organoids was immediately visualized by confocal laser scanning microscope (Olympus Fluoview FV1000, Olympus Life Science Europa GmbH, Germany)

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For example, research on absorbable acoustic microrobots has shown that using a hydrophilic/hydrophobic bilayer structure and bubble cavity design enables stable propulsion during treatment, followed by hydrolytic degradation, significantly reducing the risk of long-term residuals ( From a translational perspective, several key issues must be addressed before nanorobots can enter clinical applications: immune compatibility, visualization capabilities, degradable metabolism, dosage control, and clearance mechanisms ( in vivo must be systematically resolved and standardized ( 4.2 Translation, regulation, and ethics For micro/nanorobot technology to successfully transition from the laboratory to clinical practice, it must undergo a rigorous translation process that includes meeting various requirements, from technical performance verification to compliance with ethical and regulatory standards