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The PVDF membrane was subsequently washed (5 min 5) with tris-buffered saline with tween (TBST) buffer and then incubated with the appropriate secondary antibody (Peroxidase-conjugated goat anti-rabbit IgG (H + L), at a dilution of 1:5000) in a 5% bovine serum albumin solution for 1 h at 25 C

These findings demonstrate that VPA exposure induces significant microglial activation in the hippocampal CA1 region, which is effectively attenuated by hCiPSC-NSCs treatment, indicating a substantial reduction in neuroinflammatory responses

doi: 10.1080/19490976.2025.2517377 580 PanSHsuJCHungKTHoCJ

Those exposed to secondhand smoke are at increased risk of developing lung cancer as well