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The protein free supernatant was collected and mixed immediately with 0.5 mL of NADPH (4 mg of reduced form was dissolved in 100 mL of 0.5% NaHCO 3 ), 0.5 mL of glutathione reductase (6 units/mL in 0.1 M phosphate buffer, pH 7.0) and 1 mL of 0.6 M DTNB (prepared in 0.2 M phosphate buffer of pH 8)

[PubMed 3513553] 1113

We also thank the Biomedical Research Unit and Biomedical Research Core of Tohoku University Hospital and Biomedical Research Core of Tohoku University Graduate School of Medicine for technical support

These people are well known to have lower than normal glutathione (GSH) levels leading to their cells and tissue is suffering from chronic oxidative stress [1-5]