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World J Gastroenterol 2014

Workflow of GST Pull-down Our Advantages Setting up multiple control groups that fully exclude interference and ensure reliable results Repeated experiments (twice) to exclude random factor influences Complete downstream package for further quantitative analysis using the BLI technology Utilizing the silver staining technology with 100 times higher sensitivity than Komas Brilliant Blue to ensure clearer and better SDS-PAGE electrophoresis results One-stop service from GST protein preparation to protein interaction detection Higher affinity and specificity Protein-protein interaction (PPI) is the basis of many life activities, so the research enthusiasm for PPI has not diminished

Electron acceptors are glutathionylated disulfide substrates (GSSR) 1,2,3,10,11,12 or non-glutathione disulfide substrates such as Escherichia coli ribonucleotide reductase (RSSR) 13,14,15 (Fig