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RNA extraction and qRT-PCR The purified total RNA was extracted by Trizol reagent, and then reverse-transcribed by using the Fastking RT Kit (Tiangen, Beijing, China)

The central compartment is made up of the highly perfused tissues that equilibrate rapidly with the drug

First, proper storage: lyophilised powders should be kept sealed in a cool, dry place away from light and moisture, while reconstituted solutions require refrigeration after opening, as compound potency degrades quickly without it

J Leukoc Biol 116:13241333 Chu VT, Beller A, Rausch S, Strandmark J, Zanker M, Arbach O, Kruglov A, Berek C (2014) Eosinophils promote generation and maintenance of immunoglobulin-A-expressing plasma cells and contribute to gut immune homeostasis