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Carbamidomethylation was set as a fixed modification, whereas methionine oxidation, asparagine and glutamine deamination, and glycosylation options were set as variable modifications, according to published data ( The intact hCG analysis revealed several hCG glycoforms

The results reveal that cell lines fall robustly into four clusters numbered C1-C4 with no appreciable gain in cluster stability when increasing to higher numbers of clusters (Figure 1A and Table S1)

The instrument was operated with PBS as sheath fluid using a 488 nm Laser for excitation as well as a 502 nm long-pass filter with a 530/30 band-pass filter for specific detection of the sfGFP fluorescence

the number in the table indicates the number of unigene