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10.1016/j.jtcms.2020.10.005 520 ZhouS.-Y.GuoZ.-N.YangY.QuY.JinH

In graphs of pooled data, n values represent the entire population of cells recorded from at least three independent cultures (N)

For tumour xenograft models, 1 10 6 HCC-1806 cells or 5 10 6 PC3 cells were injected into the right fourth mammary fat pad or hind flank of athymic nude NU/J mice (The Jackson Laboratory, 002019) in 100 l sterile PBS or a 1:1 mixture of cell suspension in PBS and Matrigel (Corning, 356237) using a 29-gauge 1 ml insulin syringe

It would be worthwhile to phenotype different eosinophil subpopulations by membrane surface markers, in order to distinguish homeostatic versus inflammatory eosinophils