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That usually means advanced subjects, plateaued protocols, or longer cycles where metabolic adaptation becomes the limiting factor

To further validate that deficiency of hlh-11 truly affects endogenous lipl-3 and acs-2 expression, we used reverse transcription quantitative polymerase chain reaction (RT-qPCR) to examine lipl-3 and acs-2 transcript levels in wild-type animals and hlh-11 KOs

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