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These multicellular organoids contained spatially organized groups of DA neurons expressing markers typically found in vivo in A9 mDA (e.g., GIRK2, DAT) or A10 mDA (e.g., Calcineurin), as well as anatomically mature synapses, synaptic vesicle trafficking, release of DA in response to depolarizing stimuli, polarized membrane potential (Vm = 70 mV), voltage-dependent potassium currents, spontaneous calcium transients, spontaneous electrophysiological peacemaker activity that was reactive to the D2/D3-preferential DA agonist quinpirole via D2/D3 autoreceptor stimulation (Simao et al., 2015

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Delcourt, N., Claudepierre, T., Maignien, T., Arnich, N

The more you reduce your caloric intake, the bigger the drop in your metabolic rate and the quicker this will happen