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Toll-like receptor 4 responds to endogenous molecules during the sterile inflammatory processes such as IRI (82) and is considered as the key regulator in several ischemiareperfusion models

A practical overview is also available from the AANA on USP

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After treatment, cells were incubated with DCFDA 10 M for 45 minutes at 37C in 5% CO 2 followed by washing