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The efficiency of the proteomic approach has gained considerable attention for identifying various industrially important enzymes, stimulating its use in the discovery of novel nitrilase-metabolizing enzymes ( 5.1 Conventional culture-dependent approach The conventional culture-dependent approach includes the isolation of natural nitrilase/amidase-producing microorganisms that hydrolyse 3-cyanopyridine/nicotinamide into nicotinic acid and optimization of the conditions for nicotinic acid synthesis (Table 1)

This trend was similarly observed for proton leak measurements, in which HG-A reached the highest values, followed by HG-C, with both significantly exceeding those recorded under NG conditions

The spiral ganglion neurons showed necrosis and appeared shrunken with deeply stained nuclei with the presence of large vacuoles in the spiral ligament (Fig

Veening, J