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2.6.8 -glucan determination Considering that -D-glucan is a polymer of D-glucose units with -1,3 and -1,6 bonds, the concentration of -D-glucan in the previously lyophilized pellets was determined using a commercial kit (Enzymatic Yeast Beta-Glucan Assay kit, Megazyme International) based on the quantification of glucose concentration by an enzymatic procedure after enzymatic hydrolysis

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Recent studies have shown that in induced AP, particularly of alcoholic origin, there is a marked accumulation of LDs in the pancreatic cell cytoplasm, accompanied by mitochondrial dysfunction and activation of inflammatory and autophagic processes (62, 63)