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Description

Autoimmune-associated PTPN22 R620W variation reduces phosphorylation of lymphoid phosphatase on an inhibitory tyrosine residue

OCR levels were normalized on total protein content (g), and the activities of the complexes were calculated using the following OCR values: Complex I: OCR basal (Measurement 2) - OCR after rotenone (Measurement 4) Complex II: OCR after succinate (Measurement 6) Complex IV: OCR after ascorbate/TMPD (Measurement 10) Uncoupling assay Cells were plated 2 days before the assay, following the protocol of complex activities described above