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1984;140(2):282-286 Benseor IM, Calich AL, Brunoni AR, et al

Then, youll clone an expression construct that fuses GST to your protein of interest and purify your GST-fusion protein

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Primers, as indicated in Supplementary Table 2, were designed to amplify exons 40, 56 and 57 of Ryr2 , and validate the location of the desired mutations