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Recent Early Recovery After Surgery guidelines in adults recommend all patients be screened for EE but no such guidelines currently exist for pediatrics, since little is known about predictors associated with EE after pediatric LT

Neutrophils undergoing NET formation were labeled using antibodies against citrullinated histone H3 (Cit-H3, 1:1,000, ab5103, Abcam), MPO (1:1,000, AF3667, R&D system) or elastase (1:100, ab314916, Abcam), diluted in 1% BSA in PBS

This helps reduce the toxic burden on the liver, allowing it to function more effectively