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Current methodologies, particularly those relying on maleimide-type linkers, are often hampered by linker instability

To assess whether NCOA4 383522 was acting as a monomer or a dimer in this assay, we performed two complementary tests via BLI, probing for NCOA4 383522 binding to (GST-GATE16) 2 either in cis to monomeric NCOA4 383522 , or in trans to an apparently multimeric form (Fig 3F and G)