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Cleared lysate was loaded on a 5 ml Talon column (Cytiva) pre-equilibrated in H buffer (20 mM HEPES pH 7.5, 5% glycerol, 0.01% NP40, 1 mM -mercaptoethanol) containing 150 mM NaCl

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CRISPR-Cas9-mediated genome editing has successfully generated Brassica varieties with significantly increased glucoraphanin accumulation by modulating key genes in the pathway, including MYB28, AOP2, and BCAT4 (27, 96, 107)

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