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2a, cells were incubated with 5 mM of MitoSox Red mitochondrial superoxide indicator (M36008, Thermo Fisher Scientific) for 10 min at 37 C

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Microvascular contributions Although many studies show no change in blood flow associated with the development of diabetic neuropathy, deficiencies in the blood supply to peripheral nerves is considered a possible additional pathological mechanism of diabetic neuropathy (reviewed in REF

Upon lowering the pH to 5.0, a swift increase in fluorescence emission at 330 nm was observed, indicative of hydrazone unit hydrolysis and subsequent DOX release