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Cells were cultured in high glucose (25 mM) if not mentioned otherwise, supplemented with pyruvate to allow for NADH to NAD + recycling and pyruvate-dependent aspartate biosynthesis 77,78

The National Institutes of Health recommends the following vitamin B12 dosages for each category: Infants, Children and Teens The recommended daily vitamin B12 dosage for infants, children and teens is as follows: Birth to 6 months: 0.4 micrograms Infants 712 months: 0.5 micrograms Children 13 years: 0.9 micrograms Children 48 years: 1.2 micrograms Children 913 years: 1.8 micrograms Teens 1418 years: 2.4 micrograms Vitamin B12 is essential for many body functions in babies, children and teens, including proper growth and brain function

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2A), we cannot detect ubiquitinated unassembled uS10