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In our outpatient orthopedic setting, we use the principles of plasma therapy to address chronic tendonitis, osteoarthritis of the knee, and ligament sprains that havent responded to traditional conservative care

However, rutin is poorly soluble in water and fat, with low bioavailability after oral administration, which limits its clinical application

After 3 h drug exposure, cells were detached, washed twice with phosphate-buffered saline (PBS), dried at RT for 15 min and lysed in 100 L HNO 3 (69%, Rotipuran Supra, Carl Roth, Karlsruhe, Germany) for 1 h at RT