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If you notice the stopper surface deteriorating or if the vial no longer holds vacuum (you don't hear a slight hiss when first puncturing), replace the vial or transfer contents to a fresh sealed container

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Witkowski, M., Nemet, I., Alamri, H., Wilcox, J., Gupta, N., Nimer, N., Haghikia, A., Li, X.S., Wu, Y., Saha, P.P., Demuth, I., Knig, M., Steinhagen-Thiessen, E., Cajka, T., Fiehn, O., Landmesser, U., Tang, W.H.W., & Hazen, S.L