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This unexpected finding may be explained by the limited sensitivity of the immunohistochemistry (IHC) technique, which is generally less sensitive than western blotting or ELISA in detecting subtle changes in protein expression levels

Thorax (2016) 71(4):3789

The field of cataract surgery was transformed in 1967 when Charles Kelman introduced the phacoemulsification technique [77], commonly known as "phaco", where the cataractous lens is broken up by an ultrasonic probe, allowing aspiration through a 3-mm incision [78]

Serious Adverse Effects Overdose toxicity: Extremely high doses of alpha-lipoic acid (6,000 mg or more in a single day) can result in overdose, causing nausea, drowsiness, mental confusion, seizures, shock, organ failure, and death