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In experiments testing the reversibility of RNS-mediated PDHC inhibition, reactions were subsequently incubated with an excess of DTT (10 mM) or buffer control at room temperature for 30 min before measuring activity

In this study, it was assumed that using the auto-reduction of the hydroquinone adjacent to the quinone on doxorubicin was a sufficient indicator of the rate of oxidation of a potential redox cycle, either on the hydroquinone or the quinone after reduction