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In vitro cytotoxicity assays using SH-SY5Y (human neuroblastoma), Caco-2 (intestinal permeability), and hCMEC/D3 (human brain endothelial) cells demonstrated that nanoencapsulation significantly reduced the inherent cytotoxicity of free coumarin

The extract attenuated mRNA expression of intercellular adhesion molecule 1 (ICAM1) and chemokine (C-X-C motif) ligand (CXCL2) genes

2.3.3 Gene therapy Gene therapy presents significant potential for treating various eye diseases, including glaucoma, by targeting specific genetic factors involved in disease progression

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