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Additionally, in areas where Hindus reside, it is revered by worshippers and frequently planted next to temples devoted to Lord Shiva (3)

In the current study lowest numbers of animals and surgeries were done in a clean situation ( 2.3 Chronic constriction injury To evaluate the effect of AST on neuropathic pain, the CCI was induced according to the Bennett method ( 2.4 Experimental groups In total, 54 rats were divided into nine groups of six, including, the sham group (undergoing surgery without CCI and receiving DMSO 5%), CCI group (undergoing CCI and receiving DMSO 5%), gabapentin (positive control group, 100 mg/kg in DMSO 5%), AST-treated groups (5 and 10 mg/kg in DMSO 5%), flumazenil (FLU) (0.5 mg/kg), naloxone (NAL) (0.1 mg/kg), AST (10 mg/kg) + flumazenil (0.5 mg/kg) (AST + FLU), and AST (10 mg/kg) + naloxone (0.1 mg/kg) (AST + NAL) (Supplementary Figures 1, 2)

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