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[DOI] [PMC free article] [PubMed] [Google Scholar] 71.Ohno Y, Nara A, Nakamichi S, Kihara A

J Fam Pract

New therapeutical treatment is therefore important to develop

The inclusion bodies were washed with 25 ml of wash buffer (100 mM Tris pH 8.0, 2 mM EDTA and 0.01% v/v deoxycholate), sonicated and pelleted by centrifugation