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2.4.3 Flow cytometric analysis of stimulated PBMCs Stimulated and unstimulated PBMCs were labelled with a mixture of four conjugated surface antibodies including CD4/MaxLight405 (Cat#227418-ML405, USBiological Life Science, USA), CD8/Maxlight490 (Cat#214734-ML405, USBiological Life Science, USA), CD45RO/AlexaFlour647 (Cat# MCA2434A647, Bio-rad), and WC1/FITC (Cat# MA5-28518, Thermofisher) diluted in FACS buffer

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Several preventative measures have been established and continue to evolve, addressing AMR at international, national, community, hospital, and individual levels (Uchil et al., 2014)