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As the period of clinical manifestation of the disease generally occurs on average 20 to 38 days after the calf has contact with the pathogen, blood was collected on a fixed date of 20 days after inoculation (60 days of experiment) to maintain equal inoculation days among animals

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Furthermore, several studies have shown no correlation between CSF lactate levels and cerebral blood flow (i.e., they are unrelated to ischemia) (Brodersen and Jorgensen, The notion that lactate is not simply just a by-product of glycolysis but also a shuttle system was pioneered by Brooks (2002), and continues to be so (Laureys et al., 2014)