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These evaluations are more clinically relevant as similar tests are also regularly performed in human

Methionine and cysteine play roles in glutathione synthesis and recycling

After incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Thermo Fisher Scientific, 1:5000), the proteins were visualized using chemiluminescence reagents (29050, Engreen, San Jose, CA, USA) with a Gel Doc EZ Imager (Bio-Rad Laboratories, Hercules, CA, USA) and analyzed using the Image Lab software (Bio-Rad Laboratories)

Thioctic acid and dihydrolipoic acid are novel antioxidants which interact with reactive oxygen species