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Another important feature of COPD inflammation is the infiltration of lymphocytes, specially TCD8 + , into the lungs, defining the typical Th1 pro-inflammatory profile of COPD (Alter et al., 2020)

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Trypsin was neutralized with 500 l complete medium, the cells were resuspended and 100 l of the suspension was mixed with 6.9 ml Beckman Coulter Isoton II diluent (Fisher Scientific, NC2470899) for counting with a Beckman Coulter Z1 Coulter Cell Particle counter