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The tissues were lysed in sonication buffer (20 mM HEPES pH7.5

Some people may not be able to get enough b12 from their diet and may need to get a b12 injection

A recent study has examined the effect of cobalamin supplementation on the outcomes of treatment for feline patients with severe cobalamin deficiency and histories suggesting chronic gastrointestinal disease.5 In this study, serum concentrations of methylmalonic acid normalized following parenteral cobalamin supplementation, indicating that cobalamin deficiency was the cause of the high methylmalonic acid in serum

SPE has many advantages over traditional methods like liquid/liquid extraction, including the use of less solvent, shorter operation time, fewer procedure steps, and lower cost [6]