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Percentages of proliferative (BrdU + ) (n = 6 technical replicates representative of 2 independent experiments) ( a ) and apoptotic (Annexin V + ) (n = 3 technical replicates representative of 3 independent experiments, **p = 0.0042) ( b ) cells were determined

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The in-depth research revealed that in HCC cells treated with the single-dose irradiation, the expression levels of CSE and CBS, and the phosphorylation of p38 mitogen-activated protein kinases (MAPK) increased significantly, indicating that radiation upregulated endogenous H 2 S and p38 MAPK pathways