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49,119 Over the past 30 years, the widespread use of X-ray and Cryo-EM has facilitated the characterization of GPCR-orthosteric ligand complexes, with 657 class A, 16 class B1, 6 class B2, 19 class C, 18 class F, and 1 class T structures solved (supplementary Table 15)

For ribosome fractions, target bands were normalized to the intensity of the corresponding RPS6 (eS6) band in the same lane, whereas for whole-cell extracts, normalization was performed against the GAPDH band

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The narrow margin between the minimum requirement and the maximal legal limits of selenium, variation of selenium levels in raw materials, and influences of production processes, makes pet food formulation challenging