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Combining the easy scale-up of the DEF-CS single-cell culture system for iPSCs, the highly efficient DE induction by the STEMdiff Definitive Endoderm kit, the robust hepatic specification guided by DMSO and HGF, and the hepatocyte maturation promoted by DEX, dihexa and OSM, this improved method may allow for robust generation of homogeneous functional hepatocytes from human stem cells and opens up new possibilities for drug discovery, hepatotoxicity studies and liver regenerative therapy

Delivery method differences: Most published BPC-157 research has used injectable administration in animal subjects

To further confirm that the increased Acer2 level after hypoxia was caused by transcriptional activation, we detected the expression of Acer2 by RT-PCR and obtained similar results