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All current steps to set membrane potential and elicit action potentials were delivered through the recording pipette and controlled by Clampex 10 (Axon)

The presence of high follicle-stimulating hormone and luteinizing hormone driving elevated estradiol and testosterone indicates early maturation of the hypothalamic-pituitary-gonadal axis, which is driven by central activation of neurons

This revealed an increased resistance to CAP and EtBr in the ATP6AP1 KO cells, suggesting a common mechanism enhancing cell fitness in response to mitochondrial dysfunction (Fig