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Western blot analysis Protein sample prepared in Laemmli loading buffer was loaded onto 8% polyacrylamide gel, TGx Mini-PROTEAN 4-15% precast gel (BioRad), or Bolt Bis-Tris Plus mini-Protein Gel 4-12% (Invitrogen) and run at 100-140 constant voltage in Tris-Glycine or MES buffer for their respective gels

Regional distribution of type 2 thyroxine deiodinase messenger ribonucleic acid in rat hypothalamus and pituitary and its regulation by thyroid hormone

At very high doses, however, broadly suppressing reactive species can blunt signaling needed for adaptation, potentially altering training-like responses in muscle, immune calibration, or mitochondrial biogenesis

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