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549 To increase process throughput, disk stack centrifugation has been used to process hundreds of liters of cells for extract production and can be combined with increased dilution of the cell suspension before lysis to improve extract clarification

Given the paternal epigenome provides a number of key regulatory features that direct embryonic development, including DNA imprinting, non-coding RNAs, and histone modifications, precise epigenetic integrity of sperm may play a role in regulating mitochondrial activity and embryonic potential (Fig